Kidney Week

Abstract: PUB109

Modulation of Kidney Disorders in Type 2 Diabetes: Evaluating the Role of Probiotics in a Rat Model of Diabetic Kidney Disease

Session Information

• Publication Only

Category: Diabetic Kidney Disease

• 701 Diabetic Kidney Disease: Basic

Authors

• Silva, Eloiza Oliveira, Faculty of Medical Sciences of Santa Casa de São Paulo, Sao Paulo, SP, Brazil

Eloiza Oliveira Silva, MS, MSc

• Santos, Luciana Soares Costa, Faculty of Medical Sciences of Santa Casa de São Paulo, Sao Paulo, SP, Brazil

Luciana Soares Costa Santos, RN

• Vattimo, Maria De Fatima, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Maria De Fatima Vattimo, MS, MSc, PhD, RN

• Cabreira, Giulia Triolo, Faculty of Medical Sciences of Santa Casa de São Paulo, Sao Paulo, SP, Brazil

Giulia Triolo Cabreira

• Santos, Julia Ferreira, Faculty of Medical Sciences of Santa Casa de São Paulo, Sao Paulo, SP, Brazil

Julia Ferreira Santos

• Pinheiro, Monique Moreira, Faculty of Medical Sciences of Santa Casa de São Paulo, Sao Paulo, SP, Brazil

Monique Moreira Pinheiro

• Costa, Vitória Cecília Souza, Faculty of Medical Sciences of Santa Casa de São Paulo, Sao Paulo, SP, Brazil

Vitória Cecília Souza Costa

Background

To prevent the progression of Diabetic Kidney Disease (DRD), some strategies have been used. Currently there is Investigated the use of foods enriched with probiotics which, through their effects on microflora modulation, can contribute to glycemic control and mitigate complications of DM2 such as DRD. The objective of our study is to evaluate the effects of probiotics on the function, oxidative profile and renal histology of rats with DM2 and DRD.

Methods

Male Wistar rats were randomized into the following groups: Control (CT n=5) animals that received 0.9% saline solution (SF) (nicotinamide diluent (NA), 0.4 ml i.p. single dose) and after 15 min buffer citrate (streptozotocin diluent (STZ) at pH 4.2 and, i.p., single dose); Type 2 Diabetes Mellitus (DM2 n=5): animals received (NA; 100 mg/kg), i.p., single dose, in 0.9% SF after 15 minutes they received (STZ; 60 mg/kg), i.p., single dose ; Type 2 Diabetes Mellitus + Probiotic (DM2+P n=5): DM2 animals that received probiotic strains (bifidabacterium longum, bifidabacterium bifidum and lactobacillus rhamnosus, dose 10 10 CFU/mL); by gavage, 6 weeks. Renal function (serum creatine-CrS and inulin clearance-Clin) and redox profile were evaluated.

Results

The DM2 group demonstrated a reduction in inulin clearance, an increase in CrS and redox disorders. On the other hand, diabetic animals that received probiotics demonstrated attenuation in the deterioration of renal function perceived by an increase in Clin, a reduction in CrS and redox disorders.

Conclusion

The probiotic confirmed its renoprotective effect, providing a relevant positive effect on the morbidity of DKD in DM2 rats.