Abstract: TH-PO396
A Scalable Platform for Generating Uniform Human-Induced Pluripotent Stem Cell (hiPSC)-Derived Kidney Organoids Using Micropatterning
Session Information
- Development, Organoids, Injury, and Regeneration
October 24, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Development, Stem Cells, and Regenerative Medicine
- 600 Development, Stem Cells, and Regenerative Medicine
Authors
- Nademi, Samera, University of Washington School of Medicine, Seattle, Washington, United States
- Freedman, Benjamin S., University of Washington School of Medicine, Seattle, Washington, United States
Background
Organoids hold immense potential for modeling complex diseases, drug discovery, and regenerative medicine. However, current methods for generating organoids suffer from limitations in reproducibility and scalability, hindering their use in high-throughput applications. We present a novel approach to overcome these limitations by developing a standardized micropatterned platform for generating human induced pluripotent stem cell (hiPSC)-derived kidney organoids. Micropatterning has shown promise in improving the development and drug screening capabilities of other organoid types, such as neural organoids. However, its application to hiPSC-derived kidney organoids remains unexplored.
Methods
Here, we describe the first-of-its-kind method for generating uniform hiPSC-derived kidney organoids in various micropatterns, including linear, square, and hexagonal shapes, with customizable patterns and sizes.
Results
Our micropatterning method successfully generated hiPSC cultures in various geometries, including linear, square, and hexagonal shapes. We achieved precise control over both the pattern and size of the cultured hiPSCs, enabling customization for specific experimental needs.
Conclusion
In conclusion, our micropatterning method has established a foundation for the development of standardized hiPSC cultures. The precise control over patterning and size paves the way for tailored experiments and potentially unlocks a new era of consistent and scalable hiPSC-derived organoid generation. We now investigate if micropatterned hiPSCs differentiate into more uniform kidney organoids (size and structure) compared to conventional methods. This analysis, involving comparisons between patterned and non-patterned organoids, will determine if our approach enables large-scale production of consistent kidney organoids for disease modeling, drug screening, and potentially, future kidney regeneration.