Kidney Week

Abstract: TH-PO630

Soluble Angiopoietins in Idiopathic Nephrotic Syndrome

Session Information

• Membranous Nephropathy, FSGS, and Minimal Change Disease
  October 24, 2024 | Location: Exhibit Hall, Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Glomerular Diseases

• 1402 Glomerular Diseases: Clinical, Outcomes, and Therapeutics

Authors

• Nelson-Taylor, Sarah K., University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States

Sarah K. Nelson-Taylor, MD, PhD

• Bauer, Colin D., University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States

Colin D. Bauer

• Giannini, Courtney, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States

Courtney Giannini, MD, PhD

• Johnson, Richard J., University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States

Richard J. Johnson, MD

• Cara-Fuentes, Gabriel M., Nationwide Children's Hospital, Columbus, Ohio, United States

Gabriel M. Cara-Fuentes, MD, PhD

Background

Idiopathic nephrotic syndrome (INS) is associated with endothelial injury, but its involvement is poorly understood. To gain more insights, we measured soluble (serum) angiopoietins (Ang) 1 and 2 and soluble Tie2, as markers of blood vessel homeostasis, in children with INS.

Methods

We included 17 healthy children, 46 children with steroid sensitive INS (either unbiopsied or with minimal change disease pattern on histology) during relapse and 39 during remission. We measured soluble levels of Ang1, Ang2, and Tie2 in serum using commercially available ELISA kits. Currently, we are analyzing additional patients' samples and estimated total of 70 patients/group by november. Non-parametric tests were used to assess differences among groups and correlation between soluble levels in serum and time to achieve remission in those patients studied during remission. In vitro studies involving immortalized human glomerular cells exposed to healthy and disease sera are in process to test whether INS sera may directly stimulate glomerular endothelial cells to release these soluble biomarkers.

Results

Soluble Ang2 (Fig. 1a) and Ang1 levels were higher in patients in relapse and remission than in controls, and higher in those in relapse compared to remission. No differences were found in soluble Tie2 levels between healthy controls and patients. In patients studied during relapse (and with documented time in days to achieve remission), higher soluble Ang2 was associated with slower time to achieve remission (Fig. 1b).

Conclusion

INS is associated with alterations of markers of blood vessel homeostasis.

Funding

• Private Foundation Support