Kidney Week

Abstract: SA-PO284

Physical Exercise Reduces the Morbidity of Diabetic Rats Exposed to Polymyxin B Nephrotoxicity

Session Information

• Diabetic Kidney Disease: Basic - 2
  October 26, 2024 | Location: Exhibit Hall, Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Diabetic Kidney Disease

• 701 Diabetic Kidney Disease: Basic

Authors

• Silva, Eloiza Oliveira, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Eloiza Oliveira Silva, MS, MSc

• Victoria, Carla Djamila de Pina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Carla Djamila de Pina Victoria

• Silva, Juliana Veloso Gusmão, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Juliana Veloso Gusmão Silva

• Gonçalves, Maikol L C, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Maikol L C Gonçalves, RN

• García, Jessica Paola, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Jessica Paola García, RN

• Ferreira Vieira, Guilherme Henrique, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Guilherme Henrique Ferreira Vieira

• Alves, Mykelly Gomes, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Mykelly Gomes Alves, MSc

• Lima, Camila, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Camila Lima, PhD, RN

• Oliveira Maia, Alessandra, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Alessandra Oliveira Maia, RN

• Santos, Luciana Soares Costa, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Luciana Soares Costa Santos, RN

• Vattimo, Maria De Fatima, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

Maria De Fatima Vattimo, MS, MSc, PhD, RN

Group or Team Name

• Group of Studies on Acute Kidney Injury.

Background

Diabetic kidney disease (DKD) is a significant global health concern and the leading cause of terminal chronic kidney disease (CKD). Polymyxin B (PmB), a nephrotoxic drug, precipitates acute kidney injury (AKI) on DKD patients, elevating risk of disease progression. Physical exercise (PE) is a non-pharmacological intervention that alleviates DKD complications. The objective of this study is to evaluate the impact of PE on renal function in rats with DKD that received PmB.

Methods

Adult Wistar rats were randomized in: Citrate (CT): rats receiving streptozotocin vehicle (citrate; i.v., caudal, single dose); Citrate+PE: citrate rats subjected to daily swimming training (1 hour, 4 weeks); Diabetes Mellitus (DM): rats receiving streptozotocin (STZ, 60 mg/kg; i.v.; single dose, up to the 28th day); DM+PE: DM rats subjected to swimming training; DM+PmB: DM rats receiving PmB (4 mg/kg/day, i.p., once a day, 5 days); DM+PE+PmB: DM rats receiving PmB and subjected to PE. Renal function , renal hemodynamics , oxidative profile and renal histology were evaluated.

Results

The DM+PmB group reduced Clin, RBF and renal thiol levels, while CrS, microalbuminuria, RVR, the excretion of oxidative metabolites and tubulointerstitial injury score significantly increased. PE attenuated the deterioration of renal function in the DM groups.

Conclusion

Findings revealed that DKD rats with PE did not manifest acute DKD when exposed to PmB. These results offer an innovative insight into the interplay between PE and nephrotoxicity in DKD and the renal disease morbidity and pave the way for promising interventions in management of the DKD.