Abstract: SA-PO392
Plasma Oxalate and Microbiome Assessment in Patients on Hemodialysis
Session Information
- Hemodialysis and Frequent Dialysis - 2
October 26, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 801 Dialysis: Hemodialysis and Frequent Dialysis
Authors
- Zaidan, Nadim, Staten Island University Hospital, Staten Island, New York, United States
- Jaber, Karim, NYU Langone Health, New York, New York, United States
- Zhou, Boyan, NYU Langone Health, New York, New York, United States
- Charytan, David M., NYU Langone Health, New York, New York, United States
- Dember, Laura M., University of Pennsylvania, Philadelphia, Pennsylvania, United States
- Nazzal, Lama, NYU Langone Health, New York, New York, United States
Background
Uremic toxins accumulation in patients with end-stage kidney disease (ESKD) undergoing maintenance hemodialysis (HD) is associated with increased cardiovascular (CV) mortality. Plasma oxalate (POx) stands as a potentially significant contributor to CV complications in ESKD. Oxalate-degrading bacteria (ODB) are pivotal in oxalate metabolism, yet their prevalence and functional activity remain unexplored in ESKD patients. This pilot study explored the ODB community of ESKD patients.
Methods
Plasma (P) and fecal samples from 12 ESKD patients were obtained from the 8-week pretreatment phase of “The Microbiome and p-Inulin in Hemodialysis: A Feasibility Study” and used for our study. POx was calculated at both weeks 0 and 8 using a metabolomics platform (HPLC-MS) and colorimetric enzymatic assay. Fecal DNA from Week 1 and Week 8 were extracted using the Qiagen 96-well extraction kit and metagenomics sequencing was performed at NYU’s Genome Technology Center. The raw sequencing data was processed and analyzed using the Kraken2 as well as our bioinformatics pipeline specific to ODB.
Results
The within-person percentage change of POx between weeks 0 and 8 was 10.67% + 62.93 % and -0.67% ±9.2% using the enzymatic assay and metabolomics platform, respectively.
No significant variation between weeks 0 and 8 in both alpha and beta diversities indices highlighted microbiome stability across time for ESKD patients receiving hemodialysis. Several species were positively or negatively correlated with POx measured by the enzymatic and metabolomic assay (Table 1).
Conclusion
In conclusion, our study highlights the stability of the microbiome over time in patients with ESKD undergoing hemodialysis. Several ODB bacterial taxa are associated with POx levels in ESKD patients.
Species correlation with plasma oxalate
| Positive correlation with plasma oxalate | Negative correlation with plasma oxalate | ||
| Metabolomics Platform | Enzymatic Assay | Metabolomics Platform | Enzymatic Assay |
| Calditerrivibrio nitroreducens | Bifidobacterium breve | -Ruminococcus champanellensis -Thermoanaerobacterales bacterium -Eggerthella lenta -Gordonibacter pamelaeae -Mycoplasma conjunctivae -Muribaculaceae bacterium | Clostridiales bacterium |