Abstract: FR-PO183
Trauma of Origin: The Impact of Maternal AKI on Progeny
Session Information
- AKI: Mechanisms
October 25, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Odom, Rose, Indiana University School of Medicine, Indianapolis, Indiana, United States
- Soranno, Danielle Elise, Indiana University School of Medicine, Indianapolis, Indiana, United States
- Schwaderer, Andrew L., Indiana University School of Medicine, Indianapolis, Indiana, United States
- Starr, Michelle C., Indiana University School of Medicine, Indianapolis, Indiana, United States
- Basile, David P., Indiana University School of Medicine, Indianapolis, Indiana, United States
Background
Acute Kidney Injury (AKI) results in both short- and long-term systemic sequelae. The impact of AKI on future generations, however, has not been studied. Here, we investigate the impact AKI has on female mice and their progeny.
Methods
Wild-type, C57 female mice underwent bilateral ischemia-reperfusion AKI. Control groups included sham operated mice (no pedicle clamping) and unmanipulated controls. To confirm AKI had been obtained, serum BUN values were measured 24hrs post procedure. Two weeks after recovery, all mice underwent testing via transcutaneous Glomerular Filtration Rate (tGFR) to assess kidney function. They were then bred and allowed to deliver naturally. Serial measurements of progeny weight and length occurred at birth and then weekly through 42 days of age.
Results
Litter Size: For dams with AKI, we found a correlation between higher 24hr BUN to subsequent litter size produced. Dams with BUN’s of 90-115 produced 7 pups in a litter whereas dams with BUN’s between 140-175 produced 6 or 3 pups respectively.[SAL1]
Weight: At birth, male pups from AKI dams had lower weights than males born to control dams (p=0.02); whereas female pups had no significant changes across all groups. By day 7, all animals’ weights had “caught up” to their counterparts in other groups. By day 35, males from AKI dams had weights that surpassed those in control and sham groups (p=0.04).
Length: At birth, males from AKI dams were significantly shorter than males from control dams (p=0.005) while females from AKI-dams were significantly shorter than those from sham dams (p=0.01). Like the weight data, all animals “caught up” to their counterparts by day 7 of life, lasting through day 35. At day 42, however, both males and females born to AKI-dams surpassed animals in other groups in length (p=0.0002 in males and p=0.04 in females).
Conclusion
Litter size was significantly reduced post-AKI compared to sham and control animals, and the anthropometrics of the progeny were also significantly impacted at the time of birth. Growth was more impacted in males than females. Throughout the study, progeny from post-AKI dams did obtain “catch up” growth with sham and control progeny. Testing of neurocognitive status and nephron endowment is currently underway to elucidate any other sequelae in the progeny.