Abstract: SA-PO1197
Unveiling the Role of Transgelin as a Prognostic and Therapeutic Target of Kidney Fibrosis via a Proteomic Approach
Session Information
- CKD: Mechanisms - 3
October 26, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2303 CKD (Non-Dialysis): Mechanisms
Authors
- Kim, Kyu hong, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea (the Republic of)
- Kwon, Soie, Chung Ang University Hospital, Seoul, Seoul, Korea (the Republic of)
- Bae, Eunjin, Gyeongsang National University Changwon Hospital, Changwon, Gyeongsangnam-do, Korea (the Republic of)
- Yoo, Kyung Don, Ulsan University Hospital, Ulsan, Korea (the Republic of)
- Lee, Seong Min, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Park, Seong Joon, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Lee, Jae Wook, National Cancer Center, Goyang, Gyeonggi-do, Korea (the Republic of)
- Kim, Dong Ki, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Kim, Yon Su, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
- Yang, Seung Hee, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
Background
This study investigated the effects of kidney fibrosis and identified potential biomarkers based on the proteomic profile of human derived primary cell and animal models.
Methods
Mass spectrometry-based proteomic analysis of transforming growth factor β-induced human-derived tubular epithelial cells and kidney tissue of a 5/6 nephrectomy rat model were performed.
Results
351 differentially expressed proteins showing concurrent changes were identified. Among these, 69 proteins associated with the extracellular matrix, aging, and mitochondria were selected for gene set enrichment analysis. Network analysis of the selected proteins revealed five crucial proteins, of which transgelin showed the highest interactions with known fibrosis-related proteins. Consistent with the proteomics results, transgelin mRNA expression in the kidney tissue of the 5/6 nephrectomy model was elevated. Transgelin expression on kidney tissue gradually increased from intermediate to final fibrosis in the 5/6 Nx and unilateral ureteral obstruction mouse models. Subsequent validation in the kidney tissue and urine samples of patients with chronic kidney disease confirmed the upregulation of transgelin, particularly in the advanced stages of chronic kidney disease. Moreover, blocking transgelin in a cellular model of kidney fibrosis demonstrated its therapeutic potential.
Conclusion
In conclusion, transgelin is a promising biomarker and therapeutic target for kidney fibrosis in patients with chronic kidney disease. This study suggests transgelin as a potential noninvasive diagnostic biomarker and therapeutic intervention target for patients with chronic kidney disease. Further studies are warranted to elucidate the precise role of transgelin and its clinical utility in larger patient cohorts.