Abstract: FR-PO109
Dynamic Changes of SERPINA3 in the Serum of Patients after Cardiac Surgery and Mouse Kidneys Post Ischemia-Reperfusion Injury
Session Information
- AKI: Diagnosis and Outcomes
October 25, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 102 AKI: Clinical, Outcomes, and Trials
Authors
- Sai, Wenli, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Jiang, Yun, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Wang, Yanan, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Chen, Fei, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Huang, Lili, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Xiong, Fenfen, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Zhu, Jun, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Wu, Yuanyuan, Nantong University, Nantong, China
- Shi, Jiahai, Affiliated Hospital of Nantong University, Nantong, Nantong , China
- Yang, Bin, University of Leicester College of Life Sciences, Leicester, United Kingdom
Background
Serine proteinase inhibitor class A member 3 (SERPINA3) plays crucial roles in regulating serine proteases and various biological processes, but its involvement in acute kidney injury (AKI) remains unclear. Herein, the dynamic change of SERPINA3 in the serum of AKI patients and kidneys of AKI mice were evaluated.
Methods
The serum level of SERPINA3 was analyzed by enzyme-linked immunosorbent assay (ELISA) in patients underwent cardiopulmonary bypass at pre- and post-cardiac surgery at 0, 6, 12 hours (h) and 1,2,3 and 7day(s). In addition, amouse bilateral renal ischemia-reperfusion (IR) model was established by 30-min ischemia and followed by reperfusion for 6 h to 7 days. The expression of SERPINA3 and its localization in kidneys were also evaluated.
Results
Compared with pre-operation, SERPINA3in serum was upregulated from 6 and 12 h, and reached a statistical significance at Day 1,2,3 and 7 after operation in patients without AKI and with AKI stage II/II, and significantly increased at Day1,2 and 3 in patients with AKI stage I. Most interestingly, the level of serum SERPINA3 was peaked at Day 1 or Day 2, and decreased afterwards in both patients without AKI and AKI stage I, but remained high at Day 3 and Day 7 in patients with AKI stage II/III, which may indicate a potential of chronic progression. In addition, the expression of SERPINA3 was significantly raised in kidneys at 24 and 72 h, but gradually decreased afterwards. The immunostaining of SERPINA3 was mainly in interstitial areas and the cytoplasm and nucleus of tubular cells in IR kidneys. The staining in interstitial areas was increased at 6 h post IR, while that intubular areas was remained over 6-24 h. The level of SERPINA3 protein in IR kidneys was positively correlated with the score of tubulointerstitial damage, but was not related with serum creatinine.
Conclusion
SERPINA3 may be a potential early biomarker for IR-AKI, as well as an indicator for its chronic transition. The role of SERPINA3 in AKI and underlying mechanisms need to be further investigated.