Abstract: TH-PO237
Exploring the Impact of Hemodialysis Modalities on NETosis in Diabetic and Nondiabetic Patients
Session Information
- Hemodialysis, Hemodiafiltration, and Frequent Dialysis
October 24, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 801 Dialysis: Hemodialysis and Frequent Dialysis
Authors
- Kruzel-Davila, Etty, Galilee Medical Center, Nahariya, North, Israel
- Vdovich, Olga Olgav, Galilee Medical Center, Nahariya, North, Israel
- Remez, Lital, Galilee Medical Center, Nahariya, North, Israel
Background
Patients suffering from chronic kidney disease (CKD) and diabetes mellitus (DM) face an increased risk of developing cardiovascular complications and infections. Neutrophils are recruited to sites of infection and form Neutrophil extracellular traps, a conserved mechanism known as NETosis, function to eradicate pathogens by releasing decondensed chromatin decorated with proteins from neutrophil intracytoplasmic granules. However, when NETosis becomes dysregulated, it can potentially exacerbate the detrimental inflammatory pathways linked to complications of CKD and diabetes. Considering the higher survival rates observed among patients treated with hemodiafiltration (HDF) as opposed to hemodialysis (HD), along with the dysregulated NETosis observed in individuals with HD and DM, our objective is to investigate the impact of hemodialysis modality on NETosis in patients undergoing hemodialysis, with and without diabetes.
Methods
Twenty hemodialysis patients participated in the study, comprising 10 diabetic patients, 10 non-diabetic patients, and 10 healthy controls. Blood samples were obtained from patients undergoing hemodiafiltration (HDF) and subsequently transitioning to high flux hemodialysis (HFHD). Neutrophils were isolated from these samples and stimulated with 100 nM PMA for one hour. Subsequently, they were stained for markers of NETosis such as PAD4, PE, MPO, Histone H3, and 7AAD Viability Dye. Data acquisition was performed using a flow cytometry.
Results
Our findings reveal significantly reduced levels of NETosis activation and NETosis markers following HDF treatment compared to HD, irrespective of diabetes status (p<0.05). Furthermore, diabetic patients exhibited lower NETosis activation compared to non-diabetic patients (p=0.03, 0.01, for HD and HDF respectively).
Conclusion
The study suggests that the reduction in dysregulated NETosis may contribute to the favorable outcomes of HDF, ultimately leading to decreased mortality among hemodialysis patients.
Funding
- Private Foundation Support