Abstract: SA-PO456
Au@PDA Nanotag-Based Lateral Flow Immunoassay Platform for Highly Sensitive Screening of Pathogenic Bacteria and Fast Evaluation of Antibacterial Agents
Session Information
- Home Dialysis - 2
October 26, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 802 Dialysis: Home Dialysis and Peritoneal Dialysis
Authors
- Zhou, Fangfang, Ningbo No 2 Hospital, Ningbo, Zhejiang, China
- Luo, Qun, Ningbo No 2 Hospital, Ningbo, Zhejiang, China
Background
Peritoneal dialysis associated peritonitis (PDAP) is one of the most common complications of PD. Timely and accurate diagnosis of PDAP is the key to improving the dialysis quality and survival rate of PD patients.
Methods
We developed lateral flow immunoassays (LFIAs) , which had the merits of low cost, quick screening, and on-site detection are competitive technologies for bacteria detection, but their detection limits depend on the optical performance of the adopted nanotags. Herein, we presented a LFIA platform for bacteria detection using polydopamine (PDA) functionalized Au nanoparticles (denoted as Au@PDA) as the nanotag. The introduction of PDA could provide enhanced light absorption of Au, as well as numerous functional groups for conjugation. Small recognition molecules i.e. vancomycin (Van) and p-mercaptophenylboronic acid (PMBA) were covalently anchored to Au@PDA, and selected as the specific probes towards Gram-positive (G+) and Gram-negative (G-) bacteria, respectively. Taken Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) as the representative targets of G+ and G- bacteria, two LFA strips were successfully constructed based on the immuno-sandwich principle.
Results
The LFIA platform could quantitatively detect S. aureus and E. coli both down to 102 cfu/mL, a very competitive detection limit in comparison with other colorimetric or luminescent probes-based LFIAs. Furthermore, the proposed two strips were applied for the quantitative, accurate, and rapid screening of S. aureus and E. coli in food and human urine samples with good analytical results obtained. In addition, they were integrated as a screening platform for quick evaluation of diverse antibacterial agents within 3 hours, which is remarkably shortened compared with that of the traditional bacterial culture (over 3 days) and the common plate coating method (over 24 hours).
Conclusion
Au@PDA nanotag-based lateral flow immunoassay platform is highly sensitive screening of pathogenic bacteria and fast evaluation of antibacterial agents for peritoneal dialysis associated peritonitis.
Funding
- Government Support – Non-U.S.