Abstract: SA-PO272
Effects and Dynamics of D-alanine under Diabetic Conditions
Session Information
- Diabetic Kidney Disease: Basic - 2
October 26, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Diabetic Kidney Disease
- 701 Diabetic Kidney Disease: Basic
Authors
- Kimura, Tomonori, Osaka Daigaku, Suita, Osaka, Japan
- Sakai, Shinsuke, Osaka Daigaku, Suita, Osaka, Japan
- Mita, Masashi, Kagami Inc., Ibaraki, Japan
- Mizui, Masayuki, Osaka Daigaku, Suita, Osaka, Japan
- Isaka, Yoshitaka, Osaka Daigaku, Suita, Osaka, Japan
Background
D-Alanine, a rare enantiomer of alanine, protects from viral infections and mediates renal gluconeogenesis through the transcriptional network of circadian clock. These features of D-alanine are associated with the diabetic conditions; the aberrant circadian rhythm is a feature of diabetes, and diabetes is a worsening factor for Covid-19. This study investigated the effects and dynamics of D-alanine in the diabetic conditions.
Methods
The blood and urinary levels of D-alanine in diabetic model mice and in patients with diabetic kidney disease were measured using two-dimensional high performance liquid chromatography system. Gluconeogenetic activity of D-alanine was analysed using glucose production assay in ex vivo-cultured kidney cells. Glucose tolerance test was performed in mice treated with D-alanine.
Results
The circadian rhythm of D-alanine, which is clearly present in mice, was lost in mice model of diabetes. The urinary excretion profile of D-alanine was abnormal in patients with diabetic kidney disease. Like normal kidney, D-alanine induced gluconeogenesis in kidney from diabetic model mice. While treatment of D-alanine induced the transient increase of blood glucose level, repetitive treatment of D-alanine did not worsen the glucose tolerance.
Conclusion
D-Alanine also acts on the kidney from diabetic mice, whereas treatment of d-alanine does not worsen the diabetic conditions. D-Alanine may provide a therapeutic option for diabetes through the correction of circadian rhythm and treatment of viral infections.