Kidney Week

Abstract: FR-PO1151

Exercise Alters Peripheral Blood Mononuclear Cell Mitochondrial Respiratory Rates in Persons with CKD: Results from the ESTEEM-VIDA Pilot Randomized Clinical Trial

Session Information

• CKD: Kidney Function and Extrarenal Complications
  October 25, 2024 | Location: Exhibit Hall, Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

• 2302 CKD (Non-Dialysis): Clinical, Outcomes, and Trials

Authors

• Norman, Jennifer E., University of California Davis, Davis, California, United States

Jennifer E. Norman, MA, PhD

• Ahmadi, Armin, University of California Davis, Davis, California, United States

Armin Ahmadi, PhD

• Gertz, Erik R., USDA Agricultural Research Service, Davis, California, United States

Erik R. Gertz

• Bennett, Brian J., USDA Agricultural Research Service, Davis, California, United States

Brian J. Bennett, PhD

• Gipe, Jesse, University of California Davis, Davis, California, United States

Jesse Gipe

• Kim, Tae Youn, University of California Davis, Davis, California, United States

Tae Youn Kim, PhD, RN

• Begue, Gwenaelle, California State University Sacramento, Sacramento, California, United States

Gwenaelle Begue, PhD

• Gamboa, Jorge, Vanderbilt University Medical Center, Nashville, Tennessee, United States

Jorge Gamboa, MD, PhD

• Roshanravan, Baback, University of California Davis, Davis, California, United States

Baback Roshanravan, MD, MS, MPH, FASN

Background

Impaired muscle mitochondrial function is mechanistically linked to sarcopenia and frailty in chronic kidney disease (CKD). Exercise interventions may improve mitochondrial function and preserve physical function in this population. Assessment of mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) may provide insight into the effects of exercise on immunometabolism.

Methods

ESTEEM-VIDA is a 12-week pilot randomized clinical trial (NCT02923063) of a home-based, personalized, and video-supervised exercise intervention (EX), compared to usual care (UC), in persons with stage 3-5 non-dialysis CKD. We assessed changes in ex vivo mitochondrial respiration in intact (non-permeabilized) PBMCs. High resolution respirometry (Oroboros O2k-Fluo respirometer) was used to assess routine, leak, ATP-linked, electron transport (ET) capacity, and reserve capacity respiration rates. Linear mixed effects models were used to test changes in these mitochondrial respiration rates, comparing 12 week and baseline measurements within each group.

Results

Participants randomized to EX (n=21) had a mean eGFR of 35 ± 12 mL/min/1.73m² and mean age of 63 ± 10.3 years, compared to 32.3 ± 12 mL/min/1.73m² and 67.1 ± 8.3 years for those randomized to UC (n=9). While no changes occurred in ATP-linked respiration, significant (p<0.05) decreases were seen in routine, leak, ET capacity, and reserve capacity respiratory rates in the EX group after the intervention (Figure 1). No changes were observed in any of the measured respiratory rates in the UC group.

Conclusion

In persons with CKD, a home-based exercise intervention reduced all measured PBMC respiratory rates, with the exception of ATP-linked respiration. It could be hypothesized that ATP-linked respiration does not change due to cellular energy requirements being fixed. Further studies are needed to determine if the changes observed are related to improvements in oxidative stress and inflammation with exercise.

Figure 1: Selected PBMC mitochondiral respiration rates.

Funding

• NIDDK Support