Abstract: SA-PO443
Impact of Peritoneal Dialysis on Peritoneal Macrophage Immunometabolism and Its Relation to Systemic Inflammation and Cardiac Function
Session Information
- Home Dialysis - 2
October 26, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 802 Dialysis: Home Dialysis and Peritoneal Dialysis
Authors
- Karsten, Micky, Amsterdam UMC Locatie AMC, Amsterdam, Noord-Holland, Netherlands
- Hahn, Nico, Amsterdam UMC Locatie VUmc, Amsterdam, Noord-Holland, Netherlands
- Vree, Puck, Amsterdam UMC Locatie AMC, Amsterdam, Noord-Holland, Netherlands
- Vervloet, Marc G., Radboud Universiteit, Nijmegen, Gelderland, Netherlands
- Van den Bossche, Jan, Amsterdam UMC Locatie VUmc, Amsterdam, Noord-Holland, Netherlands
- Jakulj, Lily, Amsterdam UMC Locatie AMC, Amsterdam, Noord-Holland, Netherlands
Background
Heart failure with a preserved ejection fraction (HFpEF), is in part driven by invading macrophages and is common in peritoneal dialysis (PD) patients. The glucose rich PD-effluent (PDE) triggers peritoneal inflammation, alike to what is seen in HFpEF. However, the role of macrophages in PD-induced peritoneal injury is unknown. We hypothesize that human peritoneal macrophages (HPMs) of PD-treated patients exhibit an inflammation-driven immunometabolic profile that may ultimately aggravate damage in remote tissues such as the myocardium.
Methods
In adult PD-patients, we collected PDE-derived HPMs and peripheral blood mononuclear cells (PBMC) and performed echocardiography to measure left ventricular global longitudinal strain (LVGLS). We evaluated the impact of biobanking by comparing fluorescence-activated cell sorting (FACS) and (LPS-stimulated) IL-6 production between fresh and thawed HPMs.
We will obtain complex immunometabolic HPM and PBMC profiles by lactate, resazurin and SCENITH analysis. Phenotype will be analyzed with FACS and functionality is assessed by in vitro exposure to M1 and M2 inducers. We will relate HPM characteristics to systemic PBMC profiles and other markers of systemic inflammation and LVGLS.
Results
We successfully isolated HPMs from 16 PD-patients (mean age 61,9 (±19,3) years, median PD-vintage 9,5 [4-44,3] months and median LVGLS -17% [-18;-13]). Phenotype and functionality was evaluated both in fresh and thawed HPMs. FACS showed stable high percentages of CD14+HLA-DR+CD64+ macrophages and IL-6 production was maintained after biobanking both with and without lipopolysaccharides (LPS) stimulation.
Conclusion
We successfully isolated and biobanked HPMs from the easily accessible PDE, with maintained functionality. With the proposed protocol we will obtain in depth immunometabolic HPM profiles and relate these to cardiac function and systemic inflammation.
Panel A: No difference in IL-6 production of fresh and biobanked HPMs with or without LPS; Panel B: FACS showed no difference between fresh and biobanked HPM populations