Kidney Week

Abstract: FR-PO759

Change of Podocyte-Glomerular Basement Membrane Interaction in Glomerular Disease or Under Fluid Shear Stress

Session Information

• Glomerular Diseases: Mechanisms and Podocyte Biology
  October 25, 2024 | Location: Exhibit Hall, Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Glomerular Diseases

• 1401 Glomerular Diseases: Mechanisms, including Podocyte Biology

Authors

• Eom, Minseob, Yonsei University Wonju College of Medicine, Wonju, Gangwon-do, Korea (the Republic of)

Minseob Eom, MD, PhD

• Kim, Ji-Hee, Soonchunhyang University, Asan, Chungcheongnam-do, Korea (the Republic of)

Ji-Hee Kim, PhD

• Dang, Bao Thi Ngoc, Yonsei University Wonju College of Medicine, Wonju, Gangwon-do, Korea (the Republic of)

Bao Thi Ngoc Dang

• Yang, Jae Won, Yonsei University Wonju College of Medicine, Wonju, Gangwon-do, Korea (the Republic of)

Jae Won Yang, MD, PhD

• Cha, Seung-Kuy, Yonsei University Wonju College of Medicine, Wonju, Gangwon-do, Korea (the Republic of)

Seung-Kuy Cha, PhD

• Kim, Jae seok, Yonsei University Wonju College of Medicine, Wonju, Gangwon-do, Korea (the Republic of)

Jae seok Kim, MD, PhD

Background

The interaction between podocyte and glomerular basement membrane (GBM) determines podocyte stability. We hypothesized that the interaction between podocyte and GBM changed in response to adverse conditions, such as glomerular disease or fluid shear stress (FSS). Here, we aimed to explore the podoctye-GBM interaction in glomerular disease or under FSS through investigating changes of integrin (ITG) αVβ3, fibronectin, and uPAR .

Methods

We performed immunohistochemical staining for ITG β3, fibronectin, and uPAR in human kidney tissues obtained from patients with trauma (control) and nephrotic syndrome (NS). Furthermore, we exposed conditionally immortalized human podocyte cells to 200 rpm of FSS and observed changes in the expression of ITG β3, fibronectin, and uPAR. Lastly, we evaluated cell viability depending on the condition of dish coating.

Results

The results showed that ITG β3 expression was lower in the NS group, while the expressions of activated ITG β3, fibronectin, and uPAR were higher compared to the control (Figure 1). Furthermore, FSS increased the expressions of activated ITG β3, fibronectin, and uPAR (Figure 2). Dish coating with collagen or fibronectin improved cell viability compared to a non-coated dish under FSS.

Conclusion

ITG αVβ3 is activated in glomerular diseases or under FSS. The activation of ITG αVβ3 is associated with increased fibronectin and uPAR. The improvement of cell viability in a coated dish suggests that the interaction between extracellular matrix and activated ITG αVβ3 contributes to increasing podocyte stability.

Expressions of ITG b3, activated ITG b3, fibronectin, and uPAR between control and nephrotic syndrome groups.

Changes of activated ITG b3, fibronectin, and uPAR under fluid shear stress.

Funding

• Government Support – Non-U.S.