Abstract: SA-PO563
Myoglobin Adsorption Kinetics: An In Vitro Model
Session Information
- Bioengineering
October 26, 2024 | Location: Exhibit Hall, Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Bioengineering
- 400 Bioengineering
Authors
- Marcello, Matteo, Ospedale San Bortolo di Vicenza, Vicenza, Italy
- Lorenzin, Anna, Ospedale San Bortolo di Vicenza, Vicenza, Italy
- de Cal, Massimo, Ospedale San Bortolo di Vicenza, Vicenza, Italy
- Ramirez Guerrero, Gonzalo, Hospital Carlos Van Buren, Valparaiso, Valparaiso, Chile
- Ronco, Claudio, Ospedale San Bortolo di Vicenza, Vicenza, Italy
Background
Rhabdomyolysis is a condition in which a severe injury to the muscles leads to the release of muscle cells’ content, including myoglobin, into the blood with consequent acute renal injury. Myoglobin is a middle molecular weight uremic toxin presents a two-compartment model of distribution in the body. A possible therapeutic option is the use of high cut-off membranes but with the risk of significant loss of albumin and other proteins. The use of adsorption cartridges can overcome these limitations. Our study aimed to assess the adsorption capacity of neutral microporous styrene-divinylbenzene copolymer sorbent towards myoglobin.
Methods
Using a downscaled module of HA380 cartridge, we set up an ex-vivo circulation experiment in which 1 liter of saline solution with high concentration of myoglobin was pumped through the cartridge. We collected samples (3 mL) at different time points to measure myoglobin concentration. We calculated the removal ratio (RR) and mass adsorbed at given time points.
Results
Initial myoglobin concentration was 326,338 µg/L. Final myoglobin concentration after 4 hours of experiment was 2,042 µg/L. Removal Ratio at the end of the experiment was 99.4 % with a mass adsorption of 324.30 mg of myoglobin.
Conclusion
To our knowledge, this is the first experiment that assessed the adsorption capacity of polistyrene-divinylbenzene sorbent towards myoglobin and demonstrated high affinity to bind this molecule. Our results confirmed adsorption treatment as an effective option in rhabdomyolysis, avoiding complications associated to current dialytic treatment.
| Time (min) | Concentration in the reservoir (µg/L) | Mass in the reservoir (mg) | Mass adsorbed (mg) | RR (%) |
| 0 | 326,338 | 326.34 | 0.00 | 0.0 |
| 10 | 196.761 | 194.99 | 129.58 | 39.7 |
| 30 | 130.211 | 128.65 | 196.13 | 60.1 |
| 60 | 76.338 | 75.19 | 250.00 | 76.6 |
| 120 | 40.070 | 39.35 | 286.27 | 87.7 |
| 180 | 12.958 | 12.69 | 313.38 | 96.0 |
| 240 | 2.042 | 1.98 | 324.30 | 99.4 |
Schematic representation of the circuit utilized for the experiment. The flow rate was set at 200 mL/min.