Kidney Week

Abstract: TH-PO045

Dietary Supplementation with Bacillus Superoxide Dismutase Protects against Contrast-Induced AKI in Mice

Session Information

• AKI: Epidemiology, Risk Factors, and Prevention - 1
  October 24, 2024 | Location: Exhibit Hall, Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

• 101 AKI: Epidemiology, Risk Factors, and Prevention

Authors

• Choi, Young Eun, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Young Eun Choi, MD

• Jang, Yookyung, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Yookyung Jang, MD

• Yoon Sook, Ko, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Ko Yoon Sook, MA

• Hee Young, Lee, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Lee Hee Young, MA

• Koo, Tai yeon, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Tai yeon Koo, MD, PhD

• Oh, Sewon, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Sewon Oh, MD, PhD

• Kim, Myung-Gyu, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Myung-Gyu Kim, MD

• Jo, Sang-Kyung, Korea University Anam Hospital, Seoul, Korea (the Republic of)

Sang-Kyung Jo, MD, PhD

• Yang, Jihyun, Kangbuk Samsung Hospital, Jongno-gu, Seoul, Korea (the Republic of)

Jihyun Yang, MD, PhD

Background

Superoxide dismutase (SOD) is a metalloenzyme that play an important role in antioxidant defense against oxidative stress. Oxidative stress was known as one of the main pathophysiology in contrast-induce acute kidney injury (CI-AKI). Based on recent data showing the important role of gut dysbiosis, using probiotics could have a potential to modify systemic oxidative stress. We investigated the renoprotective potential of orally administered enteric-coated SOD protein purified from Bacillus amyloliquefaciens (SOD-BA) in contrast-induced acute kidney injury (CI-AKI) in mice.

Methods

CI-AKI model was induced with unilateral nephrectomy using 5 week-old C57BL/6 male mice. Giving oral gavage SOD-BA once a day, 48 hours before intravenous contrast injection (10mg/kg) 4 weeks after unilateral nephrectomy. Serum blood urea nitrogen (BUN), creatinine, and kidney tissue NGAL were determined.

Results

SOD-BA did not have kidney toxicity in the vehicle group. The unilateral CI-AKI model showed minimal kidney function deterioration based on creatinine level. However, the serum BUN and NGAL were strikingly increased. SOD-BA oral administration group showed decreased BUN and kidney NGAL level.

Conclusion

Prophylactic treatment of oral SOD-BA could serve as a potent antioxidant in the prevention and treatment of CI-AKI.

Funding

• Commercial Support – Department of, Biomlogic/Genofocus