Abstract: TH-PO919
High-Salt and Low-Salt Diets Can Modulate Kidney Immune Cells and Endothelial Cells
Session Information
- Health Maintenance, Nutrition, Metabolism - I
November 02, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1500 Health Maintenance, Nutrition, and Metabolism
Authors
- Lee, Kyungho, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
- Jeon, Junseok, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
- Jeon, Hojin, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
- Ko, Jihoon, Gachon University, Seongnam-si, Gyeonggi-do, Korea (the Republic of)
- Lee, Jung eun, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
- Huh, Wooseong, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
- Kim, Yoon-Goo, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
- Jang, Hye Ryoun, Samsung Medical Center, Cell and Gene Therapy Institute, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of)
Background
Kidney immune cells and endothelial cells mediate kidney ischemia-reperfusion injury and can affect kidney transplant outcomes. We aimed to study the effect of changes in dietary salt intake on kidney immune cells and endothelial cells in experimental models and kidney transplants.
Methods
We treated C57BL/6 mice with normal diet, low-salt diet, or high-salt diet and obtained kidneys after 6 weeks of allocated diets. Kidney sections were stained for CD45. To compare intrarenal sodium buffer, glycosaminoglycan (GAG) concentration was measured with kidney protein extracts. Lymphocytes isolated from kidneys were analyzed by flow cytometry. Human kidney transplant tissues obtained at 2 weeks after kidney transplantation by a protocol biopsy were stained with CD45 and CD31 and analyzed per donors’ pre-donation 24h urine sodium levels. Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) from healthy individuals and end-stage kidney disease (ESKD) patients were treated with additional sodium, and proliferation was assessed.
Results
Mice kidneys from high-salt diet group had higher kidney leukocytes (CD45+) than those from normal diet group (1.9±0.3% vs 0.9±0.1%, P=0.043). Kidney GAG level was lower in high-salt diet group than in low-salt group (41.0±3.1 vs 48.3±1.3 ng/mg, P=0.004). Both low-salt and high-salt diets increased effector-memory CD4+ T cells (normal 44.2±2.6%; low-salt 57.1±3.5%, P=0.020; high-salt 62.1±2.5% of CD4+ T cells, P=0.002) and mature B cells (70.0±1.5%; 79.1±1.4%, P=0.001; 76.7±1.6% of CD19+ cells, P=0.015), whereas decreased naïve CD4+ T cells (45.9±2.1%; 32.7±3.2%, P=0.016; 27.8±3.1% of CD4+ T cells, P=0.001) in kidneys. Human kidney transplants from donors under low-salt diet exhibited lower leukocytes (CD45+, 0.3±0.1% vs 0.9±0.3%, P=0.019) and higher capillary density (CD31+, 29.0±2.2% vs 16.1±1.8%, P=0.009) than those from donors under high-salt diet. The proliferation of iPSC-ECs from healthy individuals and ESKD patients after hypoxia was reduced under higher sodium concentrations.
Conclusion
High-salt and low-salt diets seem to exert adverse effects on kidney immune cell numbers and phenotypes as well as endothelial cells. Modulation of kidney microenvironment by modifying dietary salt intake could be a potential strategy to improve kidney transplant outcomes.