Kidney Week

Abstract: SA-PO266

Dapagliflozin Ameliorates Cisplatin-Induced Nephrotoxicity by Upregulating Nuclear Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Signaling Pathway: A Pre-Clinical Molecular Approach

Session Information

• Pharmacology: Kinetics, Genomics, Medication-Related Problems
  November 04, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Pharmacology (PharmacoKinetics, -Dynamics, -Genomics)

• 2000 Pharmacology (PharmacoKinetics, -Dynamics, -Genomics)

Authors

• Nair, Anuradha Asokan, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Anuradha Asokan Nair,

• Farook, Mohamed, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Mohamed Farook,

• Binu, Nirmal Nachiketh, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Nirmal Nachiketh Binu,

• Khan, Sofiya, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Sofiya Khan,

• Mohamed Mustafa, Mohamed Yehya, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Mohamed Yehya Mohamed Mustafa,

• Khan, Mohammed Moin, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Mohammed Moin Khan,

• Satyam, Shakta Mani, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Shakta Mani Satyam,

• Bairy, Laxminarayana Kurady, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Laxminarayana Kurady Bairy,

• Rehman, Abdul, Ras Al Khaimah Medical and Health Sciences University College of Medical Sciences, Ras Al Khaimah, Ra’s al Khaymah, United Arab Emirates

Abdul Rehman,

Group or Team Name

• Dr. Satyam's Lab - DAPA CKD Team.

Background

A dose-limiting side effect of cisplatin administration is nephrotoxicity which impairs a patient's quality of life. No potent nephroprotective agent is available to combat cisplatin-induced nephrotoxicity. This study aimed to explore the nephroprotective potential of dapagliflozin and silymarin alone and in combination against cisplatin-induced nephrotoxicity in Wistar rats.

Methods

30 adult Wistar rats were randomly divided into five groups (n=6/group): Group I- Normal control, Group II- Negative control, Group- III- Silymarin, Group IV- Dapagliflozin and Group V- Dapagliflozin + Silymarin. Nephrotoxicity was induced in Group II to Group V by administering cisplatin weekly for seven weeks. Biomarkers for kidney injury, inflammation, and oxidative stress were estimated following a histopathological examination of the kidney.

Results

Chronic kidney disease was significantly (p<0.05) demonstrated in cisplatin-intoxicated (negative) control
compared to normal control rats. Dapagliflozin alone and in combination with silymarin significantly (p<0.05) reduced serum urea, creatinine, inflammatory cytokines, and oxidative stress markers compared to negative control.

Conclusion

The present study revealed that dapagliflozin alone and in combination with silymarin ameliorates cisplatin-induced nephrotoxicity in Wistar rats via stimulating Nrf2/HO-1 signaling pathway.