Abstract: FR-PO1014
Heavy Metals Quantification in Nail Samples from the CRIC Study
Session Information
- CKD Mechanisms: From Mendel to Mars
November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2303 CKD (Non-Dialysis): Mechanisms
Authors
- Taylor, Robert M., University of New Mexico Health Sciences Center, Albuquerque, New Mexico, United States
- Baca, Justin T., University of New Mexico Health Sciences Center, Albuquerque, New Mexico, United States
- Pankratz, V. Shane, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, United States
- Yang, Wei, University of Pennsylvania, Philadelphia, Pennsylvania, United States
- Wang, Xue, University of Pennsylvania, Philadelphia, Pennsylvania, United States
- Unruh, Mark L., University of New Mexico Health Sciences Center, Albuquerque, New Mexico, United States
- Shah, Vallabh O., University of New Mexico Health Sciences Center, Albuquerque, New Mexico, United States
Background
Chronic kidney disease (CKD) affects >37 million American adults who experience high rates of cardiovascular events, kidney failure, and premature mortality. Recent reports suggest that environmental heavy metal (HM) exposure contributes to the rapid progression of kidney disease. Our overall hypothesis is that cumulative exposure to trace metals increases progression of CKD and higher metal burden will correlate with CKD risk.
Methods
We obtained matching nail samples (n=40) from 20 participants of the Chronic Renal Insufficiency Cohort (CRIC) at baseline (n=20) and 24-month follow-up (n=20). An equal number of subjects had fast progressing disease (EGRF CRIC difference between baseline and follow-up of -1 to 1) (n=10) and stable disease (EGFR CRIC difference between baseline and follow-up <= -10) (n=10). Metal concentrations were analyzed using Inductively Coupled Plasma Mass Spectrometry (ICP/MS). Mercury was measured on a Flow Injector Mercury System (FIMS), and boron was measured using ICP/Optical Emission Spectroscopy (ICP/OES).
Results
We successfully quantified 25 metalloids in all nail samples. Most metalloids had concentrations < 5 µg metalloid / g sample. Aluminum, boron, copper, iron, and zinc had concentrations between 5-80 µg/g. A trend was seen of lower concentrations in the 24-month, compared with the baseline samples. Chromium concentrations in the fast-progressing group were significantly lower at follow up (0.59 ± 0.45 µg/g) versus baseline (2.01 ± 1.64 µg/g) (p=0.036). Average total metal burden (sum of all metal concentrations) across all participants was 520 ± 72 µg/g and 327 ± 40 µg/g for the baseline and 24-month visits, respectively. At baseline, metal burden was 659 ±1006 µg/g and 382 ± 345 µg/g for subjects with fast and slow progressing CKD, respectively. At 2-year follow-up, metal burdens were 313 ± 327 µg/g and 340 ± 415 µg/g, respectively.
Conclusion
All metals we examined were above the limit of detection. Although not significant with a Wilcoxon Rank Sum Test, a trend of higher total metal burden was observed in the rapid progressing cohort, compared with stable progressing cohort.
Funding
- NIDDK Support