Kidney Week

Abstract: SA-PO1036

Urinary Sediment Podocin mRNA Excretion in Healthy Adults

Session Information

• Pathology and Lab Medicine - II
  November 04, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Pathology and Lab Medicine

• 1800 Pathology and Lab Medicine

Authors

• Fukuda, Akihiro, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Akihiro Fukuda,

• Suzuki, Miho, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Miho Suzuki,

• Kurimoto, Ryo, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Ryo Kurimoto,

• Okita, Jun, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Jun Okita,

• Uchida, Hiroki, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Hiroki Uchida,

• Kudo, Akiko, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Akiko Kudo,

• Nakata, Takeshi, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Takeshi Nakata,

• Sato, Yuji, Takachiho Town Hospital, Takachiho-cho, Japan

Yuji Sato,

• Shibata, Hirotaka, Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Yufu, Japan

Hirotaka Shibata,

Background

We have previously reported that the excretion of urinary sediment podocin (U-sed Pod) mRNA is a useful biomarker for the early diagnosis and evaluation of various glomerular diseases. However, no study has been conducted on healthy adults. In this study, we investigated the excretion of U-sed Pod mRNA in healthy adults, including the effects of gender and age.

Methods

We studied 249 healthy individuals (48 in their 20s, 52 in their 30s, 58 in their 40s, 49 in their 50s, and 42 in their 60s or older, including 40 in their 60s and 2 in their 70s; a male-to-female ratio of approximately 1:1) who received health checkups from June 2018 to March 2019 and did not have hypertension (>140/90 mmHg), diabetes (fasting blood sugar >126 mg/dL), albuminuria (U-Alb/Cre >30 mg/gCre), or chronic kidney disease (eGFR > 60 ml/min/1.73m²) and were not receiving medication. We measured the excretion of U-sed Pod mRNA in spot urine samples.

Results

No significant differences in U-sed Pod mRNA normalized to urinary creatinine (U-sed Pod mRNA/Cre) or U-sed Pod mRNA normalized to urinary sediment aquaporin 2 (U-sed AQP2) mRNA (U-sed Pod mRNA/AQP2 mRNA) were observed among the healthy adults less than 60 years of age. In addition, neither marker was significantly different between the genders. However, U-sed Pod mRNA/Cre excretion was significantly lower in subjects over 60 years of age compared to those less than 60 years (1.9 ± 0.5 M/gCre×10^-12 vs. 12.4 ± 1.4 M/gCre×10^-12, p <0.001). Furthermore, U-sed Pod mRNA excretion was not detected in approximately 30% of subjects over 60 years of age. U-sed AQP2 mRNA excretion was also significantly lower in subjects over 60 years of age, similar to U-sed Pod mRNA excretion.

Conclusion

No age or gender differences were detected in U-sed Pod mRNA/Cre or U-sed Pod mRNA/AQP2 mRNA excretion in healthy adults less than 60 years of age who could be used as normal controls.

Urinary sediment podocin mRNA/Cre, AQP2 mRNA/Cre and podocin mRNA/AQP2 mRNA

Funding

• Government Support – Non-U.S.