Abstract: FR-PO498
Phloretin Improves Ultrafiltration and Reduces Glucose Absorption During Peritoneal Dialysis in Rats
Session Information
- Peritoneal Dialysis: Current Topics
November 04, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 702 Dialysis: Home Dialysis and Peritoneal Dialysis
Authors
- Bergling, Karin, Lunds Universitet, Lund, Sweden
- Martus, Giedre, Lunds Universitet, Lund, Sweden
- Öberg, Carl Mikael, Lunds Universitet, Lund, Sweden
Background
Harmful glucose exposure and absorption remain major limitations of peritoneal dialysis. We previously showed that inhibition of sodium glucose co-transporter 2 did not affect glucose transport during peritoneal dialysis in rats. However, more recently we found that phlorizin, a dual blocker of sodium glucose co-transporter 1 and 2, was effective in reducing glucose diffusion in peritoneal dialysis, implicating either that sodium glucose co-transporter 1 inhibition or blockade of facilitative glucose channels by phlorizin metabolite phloretin would be effective in reducing glucose transport in peritoneal dialysis.
Methods
Here we tested a selective blocker of sodium glucose co-transporter 1, mizagliflozin, as well as phloretin, a non-selective blocker of facilitative glucose channels in an experimental model of peritoneal dialysis in anesthetized Sprague-Dawley rats.
Results
Intra-peritoneal phloretin treatment reduced glucose absorption by more than 30% and resulted in a more than 50% higher ultrafiltration rate compared to control animals. Sodium removal and sodium clearances were similarly improved, whereas there was no difference in the amount of ultrafiltration per mmol sodium removed. Mizagliflozin did not influence glucose transport or osmotic water transport.
Conclusion
Taken together, our present and previous results indicate that blockers of facilitative glucose channels may be a promising target for reducing glucose absorption and improving ultrafiltration efficiency.
Forest plot of phloretin effect sizes. 95% confidence intervals of phloretin effect on Isocratic, Isovolumetric and Three-pore model glucose diffusion capacity (MTAC), Three-pore model urea MTAC, ultrafiltration-rate (UF-rate) and sodium removal compared to sham group. Interval marker represent the median of the difference.
Funding
- Government Support – Non-U.S.