Kidney Week

Abstract: TH-PO823

Exercise Training Reduces Maximal Peripheral Blood Mononuclear Cell Mitochondrial Respiration While Increasing Anti-Inflammatory IL-10 in Patients With CKD

Session Information

• Health Maintenance, Nutrition, Metabolism
  November 03, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
  Abstract Time: 10:00 AM - 12:00 PM

Category: Health Maintenance‚ Nutrition‚ and Metabolism

• 1400 Health Maintenance‚ Nutrition‚ and Metabolism

Authors

• Ahmadi, Armin, University of California Davis, Davis, California, United States

Armin Ahmadi,

• Norman, Jennifer E., University of California Davis, Davis, California, United States

Jennifer E. Norman,

• Batra, Radhika, University of California Davis, Davis, California, United States

Radhika Batra,

• Badhesha, Harshanna, University of California Davis, Davis, California, United States

Harshanna Badhesha,

• Gipe, Jesse, University of California Davis, Davis, California, United States

Jesse Gipe,

• Vargas, Chenoa R., University of California Davis, Davis, California, United States

Chenoa R. Vargas,

• Chabi, Béatrice, Universite de Montpellier, Montpellier, Languedoc-Roussillon, France

Béatrice Chabi,

• Kim, Tae Youn, University of California Davis, Davis, California, United States

Tae Youn Kim,

• Jue, Thomas, University of California Davis, Davis, California, United States

Thomas Jue,

• Begue, Gwenaelle, California State University Sacramento, Sacramento, California, United States

Gwenaelle Begue,

• Gamboa, Jorge, Vanderbilt University, Nashville, Tennessee, United States

Jorge Gamboa,

• Roshanravan, Baback, University of California Davis, Davis, California, United States

Baback Roshanravan,

Background

Patients with CKD suffer from impaired physical performance. Exercise has been shown to improve physical performance in CKD, but its impact on immune cell mitochondrial function is unknown. We tested the impact of exercise on peripheral blood mononuclear cell (PBMC) mitochondrial function and their link with inflammatory biomarkers.

Methods

We performed an interim analysis of pilot randomized trial of 12 weeks of home-based, video supervised exercise intervention in CKD. Intact PBMC bioenergetics were measured using the high resolution respirometry (Oroboros O2k). We estimated basal, maximal respiration (MR) and reserve capacity (RC). Plasma IL-10 concentrations were measured using ELISA. Two-way ANOVA was used to evaluate the impact of exercise on PBMC bioenergetics. Spearman correlation was calculated for changes in log-transformed MR and IL-10 concentrations in exercise responders defined with >5% improvement in total work measured by cycle ergometry testing.

Results

Twelve participants (60.6+/-11.1 yrs) were randomized to the exercise (EX) group and 5 (64.5+/- 8.1 yrs) were randomized to the non-exercise control (CTL) group. The mean EX eGFR was 33.3+/-14.1 compared to eGFR of 37+/-3.1 of CTL. The mean MR was 10.9 (3.8) and mean RC was 8.57 (2.8). EX was associated with a marked reduction in MR and RC compared to CTL (p=0.03 and p=0.02, respectively) (Figure 1A). The change in PBMC MR inversely correlated with changes in plasma IL-10 concentration among EX responders (n=9, r=-0.83, p=0.008) (Figure 1B).

Conclusion

A decrease in PBMC MR is associated with an increase in anti-inflammatory IL-10 levels which suggest that exercise alters PBMC mitochondrial energetics in CKD. Future studies will focus on the impact of exercise on immune cell metabolic health and its immunomodulatory effects in CKD

Figure 1. The impact of exercise intervention on PBMC respiration in CKD (A). The correlation between changes in log-transformed PBMC MR and IL-10 among exercise responders (B).

Funding

• NIDDK Support