Abstract: PO0709
Exogenous Hydrogen Sulfide Protects Kidneys of Diabetic Mice from Oxidative Injuries
Session Information
- Diabetic Kidney Disease: Basic - II
November 04, 2021 | Location: On-Demand, Virtual Only
Abstract Time: 10:00 AM - 12:00 PM
Category: Diabetic Kidney Disease
- 601 Diabetic Kidney Disease: Basic
Authors
- Chen, Xueqi, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
- Xiao, Leijuan, Department of Nephrology,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
- Ren, Zhiyun, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
- Wang, Weiwan, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
- Jia, Yutao, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
- Yu, Yanting, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
- Wang, Xiaoyan, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University,Nanjing,China, Nanjing, China
Group or Team Name
- The Core Laboratory for Clinical Research
Background
Exogenous hydrogen sulfide (H2S) protects kidneys from diabetic injuries in animal models. In order to explore its mechanisms, we determined the effects of H2S donor on renal reactive oxygen species (ROS) related enzymes in diabetic mice.
Methods
Male C57Bl/6J mice (8 weeks old) were intraperitoneally injected with STZ at 50mg/kg/day for 6 days. GYY4137 (20 mg/kg/day in 6 ml of drinking water，GYY+DM group, n=5) or vehicle (6 ml of drinking water, DM group, n=4) plus 60% fat diet were fed the mice 2 weeks after the initial STZ injection when blood glucose remained high relative to background mice. The 2 groups of diabetic mice were injected with long-acting insulin (10U/kg) weekly at week 3.
Results
GYY4137 ameliorated albuminuria and hyperglycemia at weeks 8 & 10. Serum insulin and creatinine were similar in the diabetic mice. Renal morphologic structures (HE, Massson, PAS) were improved by GYY4137 at week 10 when the mice were sacrificed. Renal nitrotyrosine (protein oxidative injury marker) was decreased along with the decrease of laminin (early fibrosis marker) in GYY+DM mice relative to DM mice (western blotting). NOX2, NOX4 were lower but NOS 1,HO2, PON1,PON2 were higher in GYY+DM than those in DM group. NOS2,NOS3, NOX1 ,HO1, SOD1-3 and COX1 were similar between groups. The levels of mRNA were not in agreement with the changes in proteins with all enzymes but HO2.
Conclusion
Our findings suggest that exogenous H2S may decrease ROS production and increase ROS cleavage in kidney via the affected enzymes, thus improve the renal oxidative damage in diabetic nephropathy.