Abstract: FR-PO816
Progressive Individualized Exercise Intervention Impacted the Cecal Microbiota Composition in a Rat Model of CKD
Session Information
- Health Maintenance, Nutrition, Metabolism - II
November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1500 Health Maintenance, Nutrition, and Metabolism
Authors
- Biruete, Annabel, Purdue University, West Lafayette, Indiana, United States
- Troutman, Ashley Danielle, Indiana University Purdue University Indianapolis, Indianapolis, Indiana, United States
- Srinivasan, Shruthi, Indiana University School of Medicine, Indianapolis, Indiana, United States
- O'Neill, Kalisha, Indiana University School of Medicine, Indianapolis, Indiana, United States
- Swanson, Kelly, University of Illinois Urbana-Champaign, Urbana, Illinois, United States
- Chen, Neal X., Indiana University School of Medicine, Indianapolis, Indiana, United States
- Moe, Sharon M., Indiana University School of Medicine, Indianapolis, Indiana, United States
- Avin, Keith G., Indiana University Purdue University Indianapolis, Indianapolis, Indiana, United States
Background
Exercise interventions are recommended to improve the musculoskeletal and cardiopulmonary health in chronic kidney disease (CKD). Exercise has been shown to impact the gut microbiome, but data in CKD is limited. We have previously shown that bone health and serum biochemistry was improved following 10 weeks of wheel running and this study hypothesized that treadmill running will improve gut microbiome in the Cy/+ rat model of CKD.
Methods
We examined the following groups (n=6-8/group): 1) Normal littermates (NL); 2) CKD rats; 3) CKD + treadmill exercise (CKD+EX). The exercise intervention was performed for at a 5° grade, 50 minutes/day, 4 days/week for 10 weeks. The running speed was progressively increased if the rat was in the upper 1/3 of the belt in the final 10 minutes; maintained if in middle; and reduced if in lower 1/3. Treatments began at 22 weeks until 32 weeks of age (moderate to severe CKD, respectively). Cecal digesta was collected at euthanasia at 32 weeks of age and the V4 region of the 16S rRNA gene was sequenced and analyzed via QIIME2.
Results
Richness of the microbiota (alpha-diversity) was not impacted by CKD phenotype or exercise. The overall microbial community (beta-diversity) was not different between NL and CKD; however, CKD+EX was different from NL and CKD (PERMANOVA corrected p<0.04). There was a higher relative abundance of Bacteroides, Ruminococcus, unclassified Desulfovibrionaceae, unclassified Peptostreptococcaceae, and Prevotella (corrected p<0.05) with exercise. Taxa whose relative abundance was lowered by the exercise intervention included Lactobacillus, Akkermansia, and Blautia.
Conclusion
A personalized progressive treadmill intervention impacted the cecal microbiota. Future studies should aim at examining the impact of exercise on microbially-derived uremic toxins and outcomes in CKD.
Funding
- NIDDK Support