Abstract: FR-PO1021
Omega-3 Fatty Acid Modifies the Mitochondrial Membrane Fatty Acid and the Erythrocyte Membrane Fatty Acid in Uremic Rats
Session Information
- CKD Mechanisms: From Mendel to Mars
November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2303 CKD (Non-Dialysis): Mechanisms
Authors
- Lee, Su mi, Dong-A University, Busan, Korea (the Republic of)
- An, Won Suk, Dong-A University, Busan, Korea (the Republic of)
Background
The kidney has the second highest mitochondrial content in the human body. Fatty acids (FAs) are one of the important energy sources and main constituents of cell membranes. Higher erythrocyte membrane oleic acid contents are related to acute coronary syndrome and omega-3 FA can reduce oleic acid contents. We investigated whether omega-3 FA modifies not only erythrocyte membrane FA but also mitochondrial membrane FA of kidney in adenine-induced uremic rats.
Methods
Male Sprague-Dawley rats were fed diets containing 0.75% adenine and 2.5% protein for three weeks. Rats were randomly divided into six groups that were fed diets containing 2.5% protein and saline with cholecalciferol (3000 IU/kg/week) or omega-3 FAs (300 mg/kg/day) with cholecalciferol, and supplemented by gastric gavage for four weeks: normal control, adenine control sacrified at 3 weeks, adenine control sacrified at 5 weeks, adenine control sacrified at 7 weeks, omega-3 FAs group sacrified at 5 weeks, and omega-3 FAs group sacrified at 7 weeks. The mitochondrial isolation membrane was used for renal mitochondrial extraction. The mitochondrial and erythrocyte membrane FA contents were measured by gas chromatography.
Results
Compared to the normal controls, serum creatinine levels in adenine controls were significantly increased and improved in omega-3 FA group. Compared to the adenine controls, erythrocyte and mitochondrial membrane monounsaturated FA contents including oleic acid and arachidonic acid (AA) levels were significantly decreased in omega-3 FA group. FA compositions were similar between erythrocytes and mitochondrial membranes in each group. Monounsaturated FA contents including oleic acid, and eicosapentaenoic acid (EPA) were higher and saturated FA was lower in the mitochondrial membrane than erythrocyte membrane in adenine controls.
Conclusion
Omega-3 FA affects not only erythrocyte membrane FA but also mitochondrial membrane FA in uremic rats. Erythrocyte membrane FA contents can reflect mitochondrial membrane FA contents of the kidney.