Kidney Week

Abstract: SA-PO945

Is Abdominal Muscle the Right Parietal Peritoneal Tissue for Gene Expression Analysis?

Session Information

• Dialysis: Peritoneal Dialysis - III
  October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Dialysis

• 703 Dialysis: Peritoneal Dialysis

Authors

• Zhang, Pei, The First Affiliated Hospital of Anhui Medical University, Anhui, He Fei, China

Pei Zhang,

• Miyata, Kana N., Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, United States

Kana N. Miyata,

• Adler, Sharon G., Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, United States

Sharon G. Adler,

• Nast, Cynthia C., Cedars Sinai Medical Center, Los Angeles , California, United States

Cynthia C. Nast,

• Mapara, Hashim, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, United States

Hashim Mapara,

• La page, Janine A., Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, United States

Janine A. La page,

• Dai, Tiane, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, United States

Tiane Dai,

Background

Recently there has been increased interest in studying peritoneal membrane pathophysiology at a transcriptomic level in animal models. Standard tissue sampling includes abdominal wall muscle. Some consider muscle as part of the peritoneal barrier. However the massive muscle mass overwhelms signals from the mesothelium and sub mesothelial compact zone where much of the peritoneal ultrafiltration occurs. To address this, we performed RNAseq in microdissected parietal peritoneum without muscle compared to the samples with muscle in rats with polycystic kidneys (PCK) chronically infused with 4.25% Dianeal, with or without JAK1/2 inhibitor (JAK1/2i) or losartan for 16 wks.

Methods

PCK rats received dialysate infusions BID for 16 wks in the following groups: Control-no infusion; 4.25% Dianeal; 4.25% Dianeal + JAK1/2i (5mg/kg BID); 4.25% Dianeal + Losartan (5mg/kg BID). Parietal peritoneum was sampled either with microdissection under anesthesia without muscle or standardly with muscle. Total RNA was used, cDNA libraries were single-end sequenced (50bp) on an Illumina HiSeq 3000. Differentially expressed (DE) genes are defined as at least 2-fold change and p<0.05.Comprehensive pathway analysis was performed using Ingenuity’s Pathway Analysis (IPA).

Results

As shown (Table), there are significant differences in DE genes or pathways when using different samples with the same intervention. The peritoneum without muscle shows significantly more DE genes with more pathways involved. When comparing samples + muscle, only a few shared gene transcript changes were observed.

Conclusion

Microdisseted parietal peritoneum samples showed more DE genes and pathways than samples with muscle. Our study suggests more precise tissue sampling should be considered to better reflect peritoneal signals in transcriptomic analysis studies.

Funding

• Commercial Support – Renal research Institute