Abstract: SA-PO1019
Decreased Release of Aquaporin-2 in Urinary Extracellular Vesicles in Rats Subjected to Allogenic Kidney Transplantation
Session Information
- Fluid and Electrolytes: Basic - II
October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Fluid and Electrolytes
- 901 Fluid and Electrolytes: Basic
Authors
- Sonoda, Hiroko, University of Miyazaki, Miyazaki, Japan
- Nakanishi, Tomonori, University of Miyazaki, Miyazaki, Japan
- Kabayama, Tomoyuki, University of Miyazaki, Miyazaki, Japan
- Oshikawa, Sayaka, University of Miyazaki, Miyazaki, Japan
- Ikeda, Masahiro, University of Miyazaki, Miyazaki, Japan
Background
Diuresis has been observed within a week following renal transplantation. Aquaporin-2 (AQP2) plays an important role in the regulation of urinary concentration. Recently, AQP2 has been shown to be predominantly localized to urinary extracellular vesicles (uEVs). However, the release pattern of AQP2-bearing uEVs (uEV-AQP2) in an early phase after renal transplantation is largely unknown. In this study, we examined the release pattern of uEV-AQP2 in the recipient animals after allogenic kidney transplantation.
Methods
All animal studies were approved by the committee on the Care and Use of Laboratory Animals at the University of Miyazaki. Male SD rats were used as recipients and male Wistar rats were used as donors. The donor right kidney was transplanted into the recipient whose right kidney had been removed, and thereafter the remaining left kidney was removed (Tpx group). For the control group, a simple left kidney nephrectomy was performed. Urine and renal samples were collected at 5 days after the surgery. uEVs were isolated by differential centrifugation.
Results
Urine output was increased and urine osmolality was decreased in Tpx group in comparison with the control group. The level of renal AQP2 expression was significantly decreased in Tpx group. The release of uEV-AQP2 was significantly lower in Tpx group. Correlation analysis showed that the release of uEV-AQP2 was related to urine osmolality. When we examined marker proteins for uEVs, such as TSG101 and Alix, the release of both proteins in uEVs increased in Tpx group.
Conclusion
These data indicate that the release of uEV-AQP2 was associated with its renal expression. Furthermore, uEV-AQP2 might be an indicator for urinary concentration ability in the case of renal transplantation.