Abstract: SA-PO123
Angiopoietin-Like Protein 2 Promotes the Progression of Diabetic Kidney Disease
Session Information
- Diabetic Kidney Disease: Basic - III
October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Diabetic Kidney Disease
- 601 Diabetic Kidney Disease: Basic
Authors
- Ishii, Toshihisa, University of Yamanashi, 1110 Shimokato, Chuo,Yamanashi, Japan
- Furuya, Fumihiko, University of Yamanashi, 1110 Shimokato, Chuo,Yamanashi, Japan
- Kitamura, Kenichiro, University of Yamanashi, 1110 Shimokato, Chuo,Yamanashi, Japan
Group or Team Name
- Third Department of Internal Medicine, University of Yamanashi, JAPAN
Background
Angiopoietin-like protein 2 (ANGPTL2) is a circulating, pro-inflammatory protein. To examine the role of ANGPTL2 in the pathogenesis of diabetic kidney disease (DKD), we studied the epigenetic regulation of angptl2 expression in diabetes patients.
Methods
We determined the relationship between serum ANGPTL2 levels and the progression of DKD in cross-sectional (220 patients) and cohort (145 patients, 7-year follow-up) studies. In addition, we investigated the effect of DNA methylation on angptl2 expression and also studied the direct effect of ANGPTL2 on the podocyte function.The main outcome is the progression of DKD.
Results
Multivariate logistic regression analyses revealed that the baseline level of serum ANGPTL2 was an independent risk factor for the progression of DKD during follow-up periods. We found that DNA methylation levels of ANGPTL2 promoter in circulating monocytes were correlated with the serum ANGPTL2 levels in diabetes patients. Furthermore, in vitro, ANGPTL2 directly increased albumin permeability of the cultured podocyte through the translocation of zona occludens-1 from the membrane to the cytosol via the activation of focal adhesion kinase.
Conclusion
ANGPTL2 could be a strong mediator of DKD and serves as a good predictive biomarker for its progression.