Abstract: SA-PO174
Inflammation and Kidney Injury in Diabetic African American Men
Session Information
- Diabetic Kidney Disease: Clinical - II
October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Diabetic Kidney Disease
- 602 Diabetic Kidney Disease: Clinical
Authors
- Ongeri, Elimelda Moige, North Carolina A&T State University, Greensboro, North Carolina, United States
- Cao, Lei, North Carolina A&T State University, Greensboro, North Carolina, United States
- Jegede, Olugbemiga E., Cone Health, Greensboro, North Carolina, United States
- Harrison, Scott H., North Carolina A&T State University, Greensboro, North Carolina, United States
- Newman, Robert H., North Carolina A&T State University, Greensboro, North Carolina, United States
Background
African Americans (AA) are disproportionately burdened by diabetes and diabetic kidney disease (DKD). However, little is known about the cellular and molecular mechanisms underlying the onset and progression of DKD in this population. AA men are especially underrepresented in biomedical research. We recently reported undiagnosed kidney injury in a significant proportion of diabetic AA men served by a community clinic in Greensboro, NC. The goal of the current study was to determine the association between specific inflammation markers and kidney injury in diabetic AA men.
Methods
We recruited three groups of AA men aged 18-65 years: 1) diabetics without diagnosed kidney disease (n=87); 2) diabetics with diagnosed DKD (n=20); and 3) age-matched non-diabetic controls (n=81). Assays for urinary albumin and creatinine as well as plasma kidney injury molecule 1 (KIM-1) and urinary neutrophil gelatinase associated lipocalin (NGAL) were used for biochemical assessment of kidney function. Enzyme-linked immunosorbent assays were used to measure the plasma and urinary levels of seven inflammatory markers: adiponectin, C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), TNF receptor 1 (TNFR1), TNF receptor 2 (TNFR2), interleukin-6 (IL-6), and intercellular cell adhesion molecule-1 (ICAM-1). A heat map was generated for the “inflammation profile” for each individual in the study.
Results
Plasma levels of TNF-α, TNFR1 and TNFR2 increased with severity of DKD and were significantly higher in diabetic patients with macroalbuminuria compared to non-diabetic controls and diabetics with normoalbuminuria or microalbuminuria. Likewise, urinary levels of ICAM-1 were higher in diabetic patients with macroalbuminuria compared to the other groups. Indeed urinary ICAM-1, plasma TNF-α, and plasma adiponectin had moderate positive correlations with UACR while the levels of TNFR1 and TNFR2 in the plasma were strongly correlated with kidney injury. In contrast, though plasma CRP was elevated in diabetics relative to non-diabetic controls, its levels did not correlate with kidney injury. The number of individuals with elevated levels of multiple inflammation markers increased with the severity of kidney injury.
Conclusion
These data suggest that inflammation, particularly that mediated by the TNF-α/NF-κB signaling axis, may play a role in the pathogenesis of DKD in AA men.
Funding
- Other NIH Support