Abstract: SA-PO944
Early Peritoneal Dialysis Ameliorates Acute Lung Injury Induced by Blast Injury in Rats
Session Information
- Dialysis: Peritoneal Dialysis - III
October 27, 2018 | Location: Exhibit Hall, San Diego Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 703 Dialysis: Peritoneal Dialysis
Authors
- Chen, Kehong, Daping hospital, Chongqing, Chongqing, China
- He, Yani, Daping Hospital, Chongqing, China
Background
Blast injury frequently occurs in wars and disasters. Blast lung injury is the most common type of blast injury with a remarkable lack of effective treatment. Blast lung injury manifested as pulmonary hemorrhage, edema and secondary systemic inflammation. This study aimed to investigate the therapeutic efficacy and mechanism of peritoneal dialysis in rats with blast lung injury.
Methods
75 SD rats were randomly divided into five groups: control group, sham group, glucocorticoid (GC) group, peritoneal dialysis (PD) group, PD+GC (PDGC) group. All rats were injured by bio-shock tube (BST-I) apparatus. PD group was treated with continuous peritoneal dialysis for 6 hours. GC group were injected with a small dose of methylprednisolone. Lung function, pathological changes and pneumonedema in rats were detected after 24h treatment. Inflammatory cytokine levels in serum were determined by the technology of Milliplex.
Results
37 rats (49.3%) survived at 24 hours after blast injury. There was no significant difference in the mortality rate between the groups. Lung water content of rats in PD and PDGC groups were significantly lower than that of the control group. Pathological staining showed that the degree of pulmonary interstitial edema in PD and PDGC rats was significantly lower than that in the control group. Pulmonary function (FVC, functional residual capacity, oxygen saturation, arterial oxygen pressure, oxygenation index, and maximal mid expiratory flow) in PD and PDGC groups was significantly higher than that of the control group. The serum levels of IL-1β, IL-6, MCP-1 and TNFα in the PD and PDGC rats were significantly lower than those in the control group. The levels of IL-1β and TNFα in the serum of GC rats were significantly lower than those of the control group.
Conclusion
Early peritoneal dialysis improves acute lung injury and systemic inflammation induced by blast injury.
Funding
- Government Support - Non-U.S.